Characterization of the genetic structure of a Hereford breed herd based on STR loci
Keywords:
Microsatellites, polymorphism, genetic herd structure, locus, wright fixation index, allele, heterozygosity, hereford breed.
Abstract
The authors present a characterization of the genetic structure of a Hereford breed herd based on 15 STR loci of nucleotide sequences of the DNA. Ear tissue from 30 heifers obtained from “Bizon” LLC (Tyumen Region, Russia) was used as the material for the study. The set of markers for analysis included 15 microsatellites: BM 1818, BM 1824, BM 2113, CSRM 60, CSSM 66, ETH 3, ETH 10, ETH 225, ILST 006, INRA 023, SPS 115, TGLA 53, TGLA 122, TGLA 126, TGLA 227. To achieve the goal, the authors determined allele frequencies, polymorphism, observed and expected heterozygosity and Wright’s fixation index. The survey found that 15 microsatellite loci included 104 alleles, the frequencies of which ranged from 0.017 to 0.683. The average number of informative alleles per locus was 6.93 and the number of effective alleles was 4.96, or 71.6%. An increase in the number of alleles in the locus was accompanied by an increase in the level of polymorphism, which was confirmed by the value of the positive correlation coefficient, which was 0.794 (p < 0.001). The TGLA 53 locus had the highest polymorphism and the number of effective alleles was 10.0. The TGLA 126 locus had the lowest polymorphism and the number of effective alleles was 2.3. An analysis of genetic diversity demonstrated that the highest observed heterozygosity was registered in the TGLA 53 locus and was 0.950 and the highest expected heterozygosity was recorded in the CSSM 66 locus and was 0.860. The average level of observed heterozygosity was 0.783, the average level of expected heterozygosity was 0.691 and Wright’s fixation index was 0.133, indicating a high level of genetic diversity of Hereford herds.
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References
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Chernykh, O.U., Koshchaev, A.G., Lysenko, A.A. Shevchenko, A.A., & Mishchenko, A.V. (2017). Experience of diagnostics and containment of foot and mouth disease of cattle in Krasnodar Region, Russia. Journal of Experimental Biology and Agricultural Sciences, 6(5), 786-792.
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Hayes, B.J., Bowman, P.J., Chamberlain, A.J., & Goddard, M.E. (2009). Invited review: Genomic selection in dairy cattle: progress and challenges. Journal Dairy Sciences, 2, 433-443.
Kenijz, N., Koshchaev, A., Sokol, N., Omarov, R., & Shlykov, S. (2019). Production of bakery products from frozen dough pieces using a cryoprotectant as a yeast cell stabilizer. Indo-American journal of pharmaceutical sciences, 6(3), 6308-6315.
Kenijz, N.V., Koshchaev, A.G., Nesterenko, A.A., Omarov, R.S., & Shlykov, S.N. (2018). Study the effect of cryoprotectants on the activity of yeast cell and the moisture state in dough. Research Journal Of Pharmaceutical Biological And Chemical Sciences, 6(9), 1789-1796.
Koshchaev, A., Takhumova, O., Omarov, R., Shlykov, S., & Konik, N. (2019). Biotechnology cultivating the physiologically adapted lactobacilli to create microbial bio-additive for poultry. Indo american journal of pharmaceutical sciences, 6(3), 5735-5740.
Koshchaev, A.G., Lysenko, Y.A., Semenenko, M.P., Kuzminova, E.V., Egorov, I.A., & Javadov, E.J. (2018a). Engineering and development of probiotics for poultry industry. Asian Journal of Pharmaceutics, 12(4), 1179-1185.
Koshchaev, A.G., Shchukina, I.V., Garkovenko, A.V., Ilnitskaya, E.V., Radchenko, V.V., Bakharev, A.A., & Khrabrova, L.A. (2018b). Allelic variation of marker genes of hereditary diseases and economically important traits in dairy breeding cattle population. Journal of Pharmaceutical Sciences and Research, 10(6), 1566-1572.
Koshchaev, A.G., Shchukina, I.V., Semenenko, M.P., Krivonogova, A.N., & Vasilevich, K.V. (2016). Amino acid profile of meat of specialized beef breeds. Research Journal of Pharmaceutical, Biological and Chemical Sciences, 7(5), 670-676.
Nesterenko, A., Koshchaev, A., Kenijz, N., Luneva, A., & Varivoda, A. (2019a). Biomodification of raw meat in order to obtain functional products enriched with beneficial microflora. Indo american journal of pharmaceutical sciences, 6(3), 6347-6353.
Nesterenko, A., Koshchaev, A., Zabashta, N., Omarov, R., & Shlykov, S. (2019b). Basics of the production of protein-fat emulsions based on vegetable raw materials. Indo american journal of pharmaceutical sciences, 6(3), 6337-6346.
Nesterenko, A.A., Koshchaev, A.G., Kenijz, N.V., Akopyan, K., Rebezov, M., & Okushanova, E. (2018). Biomodification of meat for improving functional-technological properties of minced meat. Research Journal Of Pharmaceutical Biological And Chemical Sciences, 6(9), 95-105.
Nesterenko, A.A., Koshchaev, A.G., Kenijz, N.V., Shhalahov, D.S., & Vilts, K.R. (2017a). Development of device for electromagnetic treatment of raw meat and starter cultures. Research Journal of Pharmaceutical Biological And Chemical Sciences, 1(8), 1080-1085.
Nesterenko, A.A., Koshchaev, A.G., Kenijz, N.V., Shhalahov, D.S., & Vilts, K.R. (2017b). Effect of low frequency electromagnetic treatment on raw meat. Research Journal of Pharmaceutical Biological and Chemical Sciences, 1(8), 1071-1079.
Onischuk, P.D., Semenenko, M.P., Kuzminova, E.V., & Koshchaev, A.G. (2016). Selective mechanisms of antiviral effect of triazole derivatives in a transplantable virus-producing cell culture of hamadryas baboon. Research Journal of Pharmaceutical, Biological and Chemical Sciences, 7(6), 1778-1782.
Plutakhin, G.A., Koshchaev, A.G., & Donnik, I.M. (2016). Quality assessment of chicken meat by analysis-of-variance method. Research Journal of Pharmaceutical, Biological and Chemical Sciences, 7(3), 2293-2299.
Sedykh T.A., Gladyr E.A., Dolmatova I.Yu., Volkova V.V., Gizatullin R.S., & Zinoveva N.A. (2014). Polimorfizm mikrosatellitnykh lokusov krupnogo rogatogo skota gerefordskoi porody razlichnykh ekologo-geneticheskikh generatsii [Polymorphism of the microsatellite loci of the Hereford breed cattle of various ecological and genetic generations.]. Vestnik APK Stavropolya [Bulletin of the agroindustrial complex of the Stavropol Region], 3, 121-128.
Sheveleva, O.M. (2008). Proizvodstvo govyadiny na osnove spetsializirovannogo myasnogo skotovodstva [Beef production based on specialized beef cattle breeding]. Glavnyi zootekhnik [Chief livestock specialist], 11, 23-27.
Sobol, I.V., Donchenko, L.V., Rodionova, L.Y., Koshchaev, A.G., & Stepovoy, A.V. (2017). Peculiarities of analytical characteristics of pectins extracted from sunflower hearts. Asian Journal of Pharmaceutics, 11(1), S97-S100.
Starostina, N.G., Koshchaev, A.G., Ratner, E.N., & Tsiomenko, A.B. (1997). Assessment of cell-surface hydrophobicity in methanotrophic bacteria by their adherence to hydrocarbons. Microbiology, 66(2), 151-156.
Stevanovic, J., Stanimirovic, Z., Dimitrijevic, V., Stojić, V., Fratrić, N., & Lazarević, M. (2009). Microsatellite DNA polymorphism and its usefulness for pedigree verification in Simmental cattle from Serbia. Acta Veterinaria (Beograd), 5-6(59), 621-631.
Sulimova, G.E., Voronkova, V.N., Perchun, A.V., Gorlov, I.F., Randelin, A.V., Slozhenkina, M.I., & Zlobina, E.Y. (2016). Characterization of the Russian breef cattle breed gene pools using inter simple sequence repeat DNA analysis (ISSR analysis). Russian Journal of Genetics, 52, 963-968.
Tyngoziyeva, A.T., Karymsakov, T.N., & Nurbaev, S.D. (2017). Genetic structure of populations of uazakhwhiteheaded and Hereford cattles on the basis of microsatellite DNA. On-Line Journal of Biological Sciences, 17, 359-362.
Ashoory, R., Amirinia, C., Noshary, A., & Seydabadi, H. (2015). Application of eighteen microsatellite markers in studies parentage testing and genetic diversity in Holstein cattles. International Journal of Biology, Pharmacy and Allied Sciences. 4, 5823-5832.
Bakharev, A.A., Sheveleva, O.M., & Besedina, G.N. (2017). Kharakteristika i istoriya formirovaniya myasnogo skotovodstva Tyumenskoi oblasti. [Characterization and history of the formation of beef cattle breeding in the Tyumen Region.]. Mir Innovatsii [World of Innovation.], 1, 65-69.
Chasovshchikova, M.A., Sheveleva, O.M., Svjazhenina, M.A., Tatarkina, N.I., Satkeeva, A.B., Bakharev, A.A., Ponomareva, E.A., & Koshchaev, A.G. (2017). Relationship between the genetic variants of kappa-casein and prolactin and the productive-biological characteristics of cows of the black-motley breed. Journal of Pharmaceutical Sciences and Research, 9(7), 1038-1044.
Chernykh, O.U., Koshchaev, A.G., Lysenko, A.A. Shevchenko, A.A., & Mishchenko, A.V. (2017). Experience of diagnostics and containment of foot and mouth disease of cattle in Krasnodar Region, Russia. Journal of Experimental Biology and Agricultural Sciences, 6(5), 786-792.
Garkovenko, A.V., Radchenko, V.V., Ilnitskaya, E.V., Koshchaev, A.G., Shchukina, I.V., Bakharev, A.A., & Sukhanova, S.F. (2018). Polymorphism of cattle microsatellite complexes. Journal of Pharmaceutical Sciences and Research, 10(6), 1545-1551.
Gladyr, E.A., Goncharenko, G.M., Gorelov, P.V., Goryacheva, T.S., Soloshenko, V.A., Khramtsova, I.A., & Zinovieva, N.A. (2011a). Izuchenie izmenchivosti mikrosatellitov pri sozdanii novogo tipa myasnogo skota Sibiri [The study of the variability of microsatellites during the creation a new type of beef cattle in Siberia]. Dostizheniya nauki i tekhniki APK [Achievements of science and technology of the agroindustrial complex], 10, 30-32.
Gladyr, E.A., Gorelov, P.V., Maurcheva, V.N., Shahin, A.V., Chinarov, Y.I., & Zinovieva, N.A. (2011b). Otsenka rezultativnosti test-sistemy na osnove mikrosatellitov v provedenii DNK-ekspertizy krupnogo rogatogo skota [Evaluation of the effectiveness of the test system based on microsatellites in the DNA examination of cattle]. Molochnoe i myasnoe skotovodstvo [Dairy and beef cattle breeding], 8, 51-54.
Hayes, B.J., Bowman, P.J., Chamberlain, A.J., & Goddard, M.E. (2009). Invited review: Genomic selection in dairy cattle: progress and challenges. Journal Dairy Sciences, 2, 433-443.
Kenijz, N., Koshchaev, A., Sokol, N., Omarov, R., & Shlykov, S. (2019). Production of bakery products from frozen dough pieces using a cryoprotectant as a yeast cell stabilizer. Indo-American journal of pharmaceutical sciences, 6(3), 6308-6315.
Kenijz, N.V., Koshchaev, A.G., Nesterenko, A.A., Omarov, R.S., & Shlykov, S.N. (2018). Study the effect of cryoprotectants on the activity of yeast cell and the moisture state in dough. Research Journal Of Pharmaceutical Biological And Chemical Sciences, 6(9), 1789-1796.
Koshchaev, A., Takhumova, O., Omarov, R., Shlykov, S., & Konik, N. (2019). Biotechnology cultivating the physiologically adapted lactobacilli to create microbial bio-additive for poultry. Indo american journal of pharmaceutical sciences, 6(3), 5735-5740.
Koshchaev, A.G., Lysenko, Y.A., Semenenko, M.P., Kuzminova, E.V., Egorov, I.A., & Javadov, E.J. (2018a). Engineering and development of probiotics for poultry industry. Asian Journal of Pharmaceutics, 12(4), 1179-1185.
Koshchaev, A.G., Shchukina, I.V., Garkovenko, A.V., Ilnitskaya, E.V., Radchenko, V.V., Bakharev, A.A., & Khrabrova, L.A. (2018b). Allelic variation of marker genes of hereditary diseases and economically important traits in dairy breeding cattle population. Journal of Pharmaceutical Sciences and Research, 10(6), 1566-1572.
Koshchaev, A.G., Shchukina, I.V., Semenenko, M.P., Krivonogova, A.N., & Vasilevich, K.V. (2016). Amino acid profile of meat of specialized beef breeds. Research Journal of Pharmaceutical, Biological and Chemical Sciences, 7(5), 670-676.
Nesterenko, A., Koshchaev, A., Kenijz, N., Luneva, A., & Varivoda, A. (2019a). Biomodification of raw meat in order to obtain functional products enriched with beneficial microflora. Indo american journal of pharmaceutical sciences, 6(3), 6347-6353.
Nesterenko, A., Koshchaev, A., Zabashta, N., Omarov, R., & Shlykov, S. (2019b). Basics of the production of protein-fat emulsions based on vegetable raw materials. Indo american journal of pharmaceutical sciences, 6(3), 6337-6346.
Nesterenko, A.A., Koshchaev, A.G., Kenijz, N.V., Akopyan, K., Rebezov, M., & Okushanova, E. (2018). Biomodification of meat for improving functional-technological properties of minced meat. Research Journal Of Pharmaceutical Biological And Chemical Sciences, 6(9), 95-105.
Nesterenko, A.A., Koshchaev, A.G., Kenijz, N.V., Shhalahov, D.S., & Vilts, K.R. (2017a). Development of device for electromagnetic treatment of raw meat and starter cultures. Research Journal of Pharmaceutical Biological And Chemical Sciences, 1(8), 1080-1085.
Nesterenko, A.A., Koshchaev, A.G., Kenijz, N.V., Shhalahov, D.S., & Vilts, K.R. (2017b). Effect of low frequency electromagnetic treatment on raw meat. Research Journal of Pharmaceutical Biological and Chemical Sciences, 1(8), 1071-1079.
Onischuk, P.D., Semenenko, M.P., Kuzminova, E.V., & Koshchaev, A.G. (2016). Selective mechanisms of antiviral effect of triazole derivatives in a transplantable virus-producing cell culture of hamadryas baboon. Research Journal of Pharmaceutical, Biological and Chemical Sciences, 7(6), 1778-1782.
Plutakhin, G.A., Koshchaev, A.G., & Donnik, I.M. (2016). Quality assessment of chicken meat by analysis-of-variance method. Research Journal of Pharmaceutical, Biological and Chemical Sciences, 7(3), 2293-2299.
Sedykh T.A., Gladyr E.A., Dolmatova I.Yu., Volkova V.V., Gizatullin R.S., & Zinoveva N.A. (2014). Polimorfizm mikrosatellitnykh lokusov krupnogo rogatogo skota gerefordskoi porody razlichnykh ekologo-geneticheskikh generatsii [Polymorphism of the microsatellite loci of the Hereford breed cattle of various ecological and genetic generations.]. Vestnik APK Stavropolya [Bulletin of the agroindustrial complex of the Stavropol Region], 3, 121-128.
Sheveleva, O.M. (2008). Proizvodstvo govyadiny na osnove spetsializirovannogo myasnogo skotovodstva [Beef production based on specialized beef cattle breeding]. Glavnyi zootekhnik [Chief livestock specialist], 11, 23-27.
Sobol, I.V., Donchenko, L.V., Rodionova, L.Y., Koshchaev, A.G., & Stepovoy, A.V. (2017). Peculiarities of analytical characteristics of pectins extracted from sunflower hearts. Asian Journal of Pharmaceutics, 11(1), S97-S100.
Starostina, N.G., Koshchaev, A.G., Ratner, E.N., & Tsiomenko, A.B. (1997). Assessment of cell-surface hydrophobicity in methanotrophic bacteria by their adherence to hydrocarbons. Microbiology, 66(2), 151-156.
Stevanovic, J., Stanimirovic, Z., Dimitrijevic, V., Stojić, V., Fratrić, N., & Lazarević, M. (2009). Microsatellite DNA polymorphism and its usefulness for pedigree verification in Simmental cattle from Serbia. Acta Veterinaria (Beograd), 5-6(59), 621-631.
Sulimova, G.E., Voronkova, V.N., Perchun, A.V., Gorlov, I.F., Randelin, A.V., Slozhenkina, M.I., & Zlobina, E.Y. (2016). Characterization of the Russian breef cattle breed gene pools using inter simple sequence repeat DNA analysis (ISSR analysis). Russian Journal of Genetics, 52, 963-968.
Tyngoziyeva, A.T., Karymsakov, T.N., & Nurbaev, S.D. (2017). Genetic structure of populations of uazakhwhiteheaded and Hereford cattles on the basis of microsatellite DNA. On-Line Journal of Biological Sciences, 17, 359-362.
Published
2019-10-11
How to Cite
Sheveleva, O., Bakharev, A., Chasovshchikova, M., Krivonogova, A., Koshchaev, A., Dunin, I., & Plemyashov, K. (2019). Characterization of the genetic structure of a Hereford breed herd based on STR loci. Amazonia Investiga, 8(23), 278-285. Retrieved from https://www.amazoniainvestiga.info/index.php/amazonia/article/view/871
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