Investigation of Estrogen (ESR) Receptor Gene Polymorphism in Multiple Sclerosis (MS) Disease

Arash Mosarrezaii; Peyvand Mohammadi; Sayyedeh Farimah Elyasi

Arash Mosarrezaii Department of Neurology, Imam Khomeini Hospital, Urmia University of Medical Sciences, Urmia
Peyvand Mohammadi Urmia University of Medical Sciences, Urmia
Sayyedeh Farimah Elyasi Urmia University of Medical Sciences, Urmia, Iran

Keywords: Multiple sclerosis, estrogen receptor, polymorphism, PCR-RFLP

Abstract

MS is the disease of the central nervous system in which myelin sheath of the optic nerves is degraded. According to the role of estrogen in the embryonic distinctions and that the estrogen is a female sex hormone and considering the high prevalence of MS in women and in regard of the fact that the sex hormones carry out their effects using their own specific receptors, the present study aims at determining the role of estrogen receptor gene in patients with MS in Azerbaijan-e-Gharbi Province and comparing the gen in both the patients and the healthy individuals so as to make it clear what role do estrogen and its receptor genes play in MS disease.
The present study is a case-control study that was conducted on MS patients who had referred to Orumia’s Imam Khomeini (may Allah consecrate the soil of his tomb) Hospital. The study sample volume was consisted of two groups: one group comprised of 30 women with MS and another group composed of 30 healthy women who had been randomly assigned to these groups. Blood samples, 2cc, were taken from all the patients following which the DNAs of the patients were extracted and the estrogen hormone receptor gene, named ESR1, was subjected to examination based on RFLP method. Next, the information was analyzed using SPSS software, version 21.
In patients with MS, eight cases (26.7%) had homozygous gene, five cases (16.6%) had heterozygous gene and 17 cases (56.7%) had healthy gene; in the healthy individuals of the control group, zero cases had homozygous gene, 3 cases (10%) had heterozygous gene and 27 cases (90%) had healthy genes.
The genotypic and allelic frequencies of Era rs2234693 (C/T) polymorphism shows a significant difference between the patient and control groups.

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Author Biographies

Arash Mosarrezaii, Department of Neurology, Imam Khomeini Hospital, Urmia University of Medical Sciences, Urmia

Department of Neurology, Imam Khomeini Hospital, Urmia University of Medical Sciences, Urmia

Peyvand Mohammadi, Urmia University of Medical Sciences, Urmia

Urmia University of Medical Sciences, Urmia

Sayyedeh Farimah Elyasi, Urmia University of Medical Sciences, Urmia, Iran

MD, Urmia University of Medical Sciences, Urmia, Iran

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